Isolation and characterization of intergeneric somatic hybrids in the Apocynaceae family
Identifieur interne : 003B66 ( Main/Exploration ); précédent : 003B65; suivant : 003B67Isolation and characterization of intergeneric somatic hybrids in the Apocynaceae family
Auteurs : I. Kostenyuk [Russie] ; O. Lubaretz [Russie] ; N. Borisyuk [Russie] ; V. Voronin [Russie] ; J. Stöckigt [Allemagne] ; Y. Gleba [Russie]Source :
- Theoretical and Applied Genetics [ 0040-5752 ] ; 1991-10-01.
Abstract
Summary: Protoplasts were isolated from callus cultures of Rauwolfia serpentina Benth., Rhazya stricta Decaisne, and Catharanthus roseus (L.) G. Don, or from leaves of Vinca minor L. Protoplast isolation, culture, and fusion techniques as well as hybrid screening systems were developed for these species, and hybrids were obtained. Hybrid combinations were Rauwolfia + Vinca, Rauwolfia + Catharanthus, Rauwolfia + Rhazya, and Catharanthus + Vinca. For hybrid isolation, the physiological complementation method was utilized. Analyses of the material obtained included a cytogenetic study of the chromosomes, a study of multiple molecular forms of transferases and esterases, and the blot hybridization of restricted nuclear DNA using ribosomal DNA as a probe. Hybrids were identified in all species' combinations tried. A ten-fold increase in the accumulation of raucaffricine (relative to the parental Rauwolfia strain) was observed in one cell line of the Rauwolfia + Vinca hybrid. Our studies indicated the genetic stability of the great majority of the hybrid cell lines over a period of more than 20 months of in vitro growth. No shoot morphogenesis has so far been observed in this material.
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DOI: 10.1007/BF00227315
Affiliations:
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Stöckigt</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute für Pharamazie, Universität Mainz, Staudingerweg 5, W-6500, Mainz</wicri:regionArea><wicri:noRegion>Mainz</wicri:noRegion><wicri:noRegion>Mainz</wicri:noRegion></affiliation></author><author><name sortKey="Gleba, Y" sort="Gleba, Y" uniqKey="Gleba Y" first="Y." last="Gleba">Y. Gleba</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Cell Biology and Genetic Engineering, Lebedevastr. 1, 252143, Kiev</wicri:regionArea><wicri:noRegion>Kiev</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Theoretical and Applied Genetics</title><title level="j" type="sub">International Journal of Plant Breeding Research</title><title level="j" type="abbrev">Theoret. Appl. Genetics</title><idno type="ISSN">0040-5752</idno><idno type="eISSN">1432-2242</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1991-10-01">1991-10-01</date><biblScope unit="volume">82</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="713">713</biblScope><biblScope unit="page" to="716">716</biblScope></imprint><idno type="ISSN">0040-5752</idno></series><idno type="istex">AAD2DE6A940675A9D5511AC39829D85C805BAAA4</idno><idno type="DOI">10.1007/BF00227315</idno><idno type="ArticleID">BF00227315</idno><idno type="ArticleID">Art8</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0040-5752</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: Protoplasts were isolated from callus cultures of Rauwolfia serpentina Benth., Rhazya stricta Decaisne, and Catharanthus roseus (L.) G. Don, or from leaves of Vinca minor L. Protoplast isolation, culture, and fusion techniques as well as hybrid screening systems were developed for these species, and hybrids were obtained. Hybrid combinations were Rauwolfia + Vinca, Rauwolfia + Catharanthus, Rauwolfia + Rhazya, and Catharanthus + Vinca. For hybrid isolation, the physiological complementation method was utilized. Analyses of the material obtained included a cytogenetic study of the chromosomes, a study of multiple molecular forms of transferases and esterases, and the blot hybridization of restricted nuclear DNA using ribosomal DNA as a probe. Hybrids were identified in all species' combinations tried. A ten-fold increase in the accumulation of raucaffricine (relative to the parental Rauwolfia strain) was observed in one cell line of the Rauwolfia + Vinca hybrid. Our studies indicated the genetic stability of the great majority of the hybrid cell lines over a period of more than 20 months of in vitro growth. 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